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striction enzyme stui  (New England Biolabs)


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    Structured Review

    New England Biolabs striction enzyme stui
    Striction Enzyme Stui, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 646 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/striction+enzyme+stui/StuI/pm41819105-717-3-6
    Average 96 stars, based on 646 article reviews
    striction enzyme stui - by Bioz Stars, 2026-09
    96/100 stars

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    Mutagenesis:

    Article Title: Pluripotent stem-cell-based screening uncovers sildenafil as a mitochondrial disease therapy.
    Article Snippet: 27 Genomic DNA was isolated using the Nucleo-Spin Tissue kit (Macherey-Nagel). .. We used re- striction enzyme StuI (NEB, R0187, 10,000 units/ml) for the m.9185T>C mutation (wild-type: 24+90 pb, mutation: 114 pb), HpaII (NEB, R0171, 10,000 units/ml) for m.8993T>C and m.8993T>G (wild type: 25+155 bp; mutant: 180 bp), XbaI (NEB, R0145, 10,000 units/ml) for m.9176 T>G (wild type: 24+155 bp, mutant: 179 bp). .. The percentage of cleaved versus uncleaved fragments was determined by capillary electrophoresis and laser detection of the FAM-labelled RFLP-fragments using the 3,500 Series Genetic Analyzer (Applied Biosystems, RRID:SCR_021901) and normalized to a standard curve of known degrees of heteroplasmy.



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    96
    New England Biolabs striction enzyme stui
    Striction Enzyme Stui, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/striction+enzyme+stui/StuI/pm41819105-717-3-6
    Average 96 stars, based on 1 article reviews
    striction enzyme stui - by Bioz Stars, 2026-09
    96/100 stars
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